Tkinter application with Splash screen + One Directory mode breaks Tk.iconphoto() in packaged app.

Description of the issue

When all of the following apply:

• Windows
• One Directory mode
• Splash screen

Then the packaged application ignores calls to Tk.iconphoto() and displays a feather icon.

Remove the splash screen, or build in One File mode, and Tk.iconphoto() works as expected.

Context information (for bug reports)

• Output of pyinstaller --version: 6.19.0

• Version of Python: 3.13.12

• Platform: Windows

• How you installed Python: Windows store

• Did you also try this on another platform? Yes, unknown.

• try the latest development version, using the following command:

pip install https://github.com/pyinstaller/pyinstaller/archive/develop.zip

• follow all the instructions in our "If Things Go Wrong" Guide (https://github.com/pyinstaller/pyinstaller/wiki/If-Things-Go-Wrong) and

Make sure everything is packaged correctly

• start with clean installation
• use the latest development version
• Run your frozen program from a command window (shell) — instead of double-clicking on it
• Package your program in --onedir mode
• Package without UPX, say: use the option --noupx or set upx=False in your .spec-file
• Repackage you application in verbose/debug mode. For this, pass the option --debug to pyi-makespec or pyinstaller or use EXE(..., debug=1, ...) in your .spec file.

A minimal example program which shows the error

import tkinter as tk

def main():
    root = tk.Tk()
    root.title("Icon Test")
    root.geometry("300x200")

    gif_data = """R0lGODlhEAAQAKUAAGRiZLS2tIyKjOTi5HR2dJyenMzKzPT29GxubJSWlISChNTW1Ozq7KyurGxq
bMTCxJSSlHx+fKSmpNTS1Pz+/GRmZIyOjOTm5Hx6fKSipMzOzPz6/HRydJyanISGhNza3Ozu7MTG
xP///wAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACH5BAEAACIALAAAAAAQABAAAAZ4QJFw
KNociEikxpBsigoJJ3LDIRylQg2Co8EKCw5OJhlSRBQKD2dLgEAS8K4WAKg4HGwCRrEYggQVdggI
egQdIEgUDXgEHFUBFE0LeGwYE04Sd2FVDU0UHBUcDyEYHAqRSB8AChdCDBYYH0kSEhtEGwGdSANO
rUJBADs="""

    # Create a PhotoImage object from the embedded data.
    icon_image = tk.PhotoImage(data=gif_data)

    # Set the window icon using iconphoto.
    root.iconphoto(True, icon_image)

    root.mainloop()

if __name__ == "__main__":
    main()

Stacktrace / full error message

N/A

Please also see https://github.com/pyinstaller/pyinstaller/wiki/How-to-Report-Bugs for more about what would use to solve the issue.